Coding

Part:BBa_K2121002

Designed by: Jinny van Doorn   Group: iGEM16_Yale   (2016-10-20)


Lambda_Beta_B5ZVM8_RHILW (red_rhizo-MAGE)

Coding sequence for beta recombinase protein homolog. For use in red recombineering (eg Multiplex Automated Genome Engineering, MAGE) of Rhizobia species (S. meliloti and CIAT 899). Identified with BLASTp from homology to the lambda coliphage beta recombinase. Derived from phage that selectively infects rhizobia spp (R. leg).

Within Yale iGEM 2016, known as gBlock 2, amplified for cloning with primers priG 449, 450. Uniprot KB B5ZVM8_RHILW. E-value 0.00041

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 871
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 252
    Illegal NgoMIV site found at 430
    Illegal NgoMIV site found at 601
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 769


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Categories
Parameters
None